D.rerio_fig4b_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------PTA----------------A---------------VIRGVQKLVLYETRARYFLVGSNNAQTKHRVLKIDRTEPRDLVIIDDKHVYSQQEVRELLGRLDLGNRTKMGQKGSS---GLSRAVSAYGIVGFVRFLEG----YYIVLITKRRKMADIG-GHSIYKIEDTNMIYIPNDSVRVT--------------------HPDEARY----------------------------------------VRIFQNVDLSSNFYFSYSYDVSHSLQYNLTLLQSWSVCVESESG-------AAGRQDTFDIFEEEGLPTQV-----VHGLSHEPYSKYVWNLRLLEKVKE---IVHPDWLLYIIHGFCGQSSILLHS-------------------------D------SARHSCMFLHRN---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------FS---------KKVSIF-MQN-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------DSKSI--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------S-------------------HKKVKK----------------------* D.rerio_fig4a_polyphosphoinositide_phosphatase_isoform_X1 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KREKRKHEKILSEELYRAITNLNQFLPPEHNIEYIAWDMARYTKSK-LCNVLDRLSMIAESVVKRTGFFVNRPDFYCHSLRPDERWGDLGGTVTPNGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYALGMIDKPK-LQFDTDCVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDAINLF-LQVFQPSETK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHSNTMTLPHHRHSYTHWWTGGVLTYLPLPFDEA---------------------------------------------QCEENMKKVS-VK-------RVNRYEESTDIYTEFFKPYELTAFDDTFSISMTNSARDFMPKTVGLDPSPF--------------------------------------------------------------------------------------------T---VRKPDESAKS-VLGSK------------------------SSKEEAVLQR--KTAASAPPPPTEE----QISSSSEDDSEDDREDDA--------SASQRSTP-------IRL-TETSH-------------------------------------------------EDPGPQ------------------------------------------------------QRL----------------PA--------------------------------------ARET----------YGL--NLLASPPDED----------ILIY-HRFAHLGQNQHKVERHDD-------------------------------------M---K-----------------------------------KS--DAVIS-LAPVSVFASDSIYEVCAPEVDVQSRNLFE-THVQTARGWVRTLGQEDVLM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYM----------------------* E.caballus_FIG4_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------TAAA---------------P---------------IISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVIIDDRHVYTQQEVRELLGRLDLGNRTKMGQKGSS---GLFRAVSAFGVVGFVRFLEG----YYIVLITKRRKMADIG-GHAIYKIEDTNMIYIPNDSVRIS--------------------HPDEARY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETT--------QTRQESFDIFEDEGLITQGGS--GVFGICSEPYMKYVWNGELLDIIKN---AVHRDWLLYIIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVMYLNQFLPPEHTIIYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGCVIPTGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLF-LGVFHPTEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTMRLLPTRRSYTYWWTPEVIKHLPLPYDEV---------------------------------------------ICAANLKKLI-VK-------KFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARDFMPKTVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VLGNK------------------------SNRDEAVLQR--KTAASAPPPPSEE----AASSSSEDDSGTDREEEG--------SVSQRSTP-------VKM-ADSGD-------------------------------------------------SAKVTE------------------------------------------------------NVV----------------QP--------------------------------------MKEV----------YGI--NLSDGLLEED----------LSIY-TRFVQLGQSQHRQDKSSQ-------------------------------------QLYSR-----------------------------------CP--DGVIK-LTPISAFSQDNIYEVQPPRVDRKSTEIFQ-AHIQAGQGITQPLGKEDTSM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYL----------------------* F.catus_FIG4_polyphosphoinositide_phosphatase_isoform_X1 M----------------------------------------P--------------------------------AAAA---------------P---------------IISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVIIDDRHVYTQQEVRELLGRLDLGNRTKMGQKGSS---GLFRAVSAFGVVGFVRFLEG----YYIVLITKRRKMADIG-GHAIYKVEDTNMIYIPNDSVRIT--------------------HPDEARY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSETT--------QTRQESFDIFEDEGLITQGGS--GVFGICSEPYMKYVWNGELLDIIKN---TVHRDWLLYIIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIIYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGCVIPTGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLF-LGVFHPTEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTMRLLPTRRSYTYWWTPEVIKHLPLPYDEV---------------------------------------------ICAANLKKLI-VK-------KFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARDFMPKTVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VLGNK------------------------SNREEAVLQR--KTAASAPPPPSEE----AVSSSSEDDSGTDREEEG--------SVSQRSTP-------VKM-TDTGD-------------------------------------------------STKVTE------------------------------------------------------NVV----------------QP--------------------------------------MKEV----------YGI--NLSDGLSEED----------LSIY-SRFVQLGQSQHKQDKSSQ-------------------------------------QLCSR-----------------------------------CP--DGVIK-LTPISAFSQDNIYEVQPPRVDRKSTEIFQ-AHIQASQGIMQPLGKEDTSM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYL----------------------* H.sapiens_FIG4_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------TAAA---------------P---------------IISSVQKLVLYETRARYFLVGSNNAETKYRVLKIDRTEPKDLVIIDDRHVYTQQEVRELLGRLDLGNRTKMGQKGSS---GLFRAVSAFGVVGFVRFLEG----YYIVLITKRRKMADIG-GHAIYKVEDTNMIYIPNDSVRVT--------------------HPDEARY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEMLKSEMT--------QNRQESFDIFEDEGLITQGGS--GVFGICSEPYMKYVWNGELLDIIKS---TVHRDWLLYIIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANCEGDVANEVETEQILCDASVMSFTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHVAALHFDQMFQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHTIVYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGCVIPTGRLQTGILRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLF-LGVFHPTEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTMRLLPTRRSYTYWWTPEVIKHLPLPYDEV---------------------------------------------ICAVNLKKLI-VK-------KFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTSSARDFMPKTVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VLGNK------------------------SNREEAVLQR--KTAASAPPPPSEE----AVSSSSEDDSGTDREEEG--------SVSQRSTP-------VKM-TDAGD-------------------------------------------------SAKVTE------------------------------------------------------NVV----------------QP--------------------------------------MKEL----------YGI--NLSDGLSEED----------FSIY-SRFVQLGQSQHKQDKNSQ-------------------------------------QPCSR-----------------------------------CS--DGVIK-LTPISAFSQDNIYEVQPPRVDRKSTEIFQ-AHIQASQGIMQPLGKEDSSM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYL----------------------* C.mydas_FIG4_polyphosphoinositide_phosphatase_isoform_X1 M----------------------------------------P--------------------------------AGAAS--------------P---------------IIRCVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPTDLVIIDDKHVYTQQEVRELLGRLDLGNRTKIGQKGSS---GLSRAVSAFGVVGFVRFLEG----YYIVLITKRRKMADIG-GHAIYKIEDTNMIYIPNDSVRVT--------------------HPDEARY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTILRMPLEILKTETT--------QTRQESFDIFEDEGLTTQGGS--GVFGICSEPYKKYVWNGQLLDIVKN---TVHHDWLLYVIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANSEGDVANEVETEQILCDASVMSFSAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHIAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVGAVTYLNQFLPPEHAIVYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRPDSYCSILRPDEKWNELGGFVVPTGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINVF-LGVFQPAEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTMSLSQTRRSYTYWWTRGVLNHLPLPYDEV---------------------------------------------TCTENIRKLI-VK-------KFHKYEEEIDIHNEFFRPYELSSFDDTFCLAMTNSIRDFMPKNVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VVGNK------------------------SNREETVLQR--KTAASAPPPPSEE----AVSSSSEDDSGTDKEDEG--------AVSQRSTP-------VKM-ADTGD-------------------------------------------------NIKITE------------------------------------------------------NIV----------------QS--------------------------------------MKDI----------YGI--NLSDVPSEDD----------LTIY-TRFVQLGQNQHKQDKSSQ-------------------------------------QLCSK----------------------------------HHL--DGVIN-IIHISSFSQDNIYEVQPPKVDKKSIDIFH-AHIQAGRGIMQPLVKEDILM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYM----------------------* G.gallus_FIG4_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------AAAAA--------------P---------------LISSVQKLVLYETRARYFLVGSNPAETKYRVLKIDRTEPKDLVIIDDKHVYTQQEVRELLGRLDLGNRTKIGQKGSS---GLSRAVSAFGIVGFIRFLEG----YYIVLITKRRKMADIG-GHAIYKIEDTNMIYIPNDSVRVT--------------------HPDEARY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEILKTETT--------QTRQESFDIFEDEGLSTQGGS--GVFGICSKPYEKYVWNGKLLEAVKN---TVHHDWLLYIIHGFCGQSKLLIYG-------------------------RPIYVTLIARRSSKFAGTRFLKRGSNSEGDVANEVETEQILYDASVMSFSAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHIAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHAIIYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRPDSYCNVLRPDEKWNELGGCVVSTGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQESINLF-LGVFKPVEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTLSLSQTRRSYTYWWTTGVLKHLPLPFDEV---------------------------------------------TCAENRRKLT-VK-------KFHKYEEEIDIHNEFFRPYELSSFDETFCLAMTNSTRDFMPKNVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VLGNK------------------------SNREETVLQR--KTAASAPPPPSEE----AVSSSSEDDSGTDKEDEG--------AVSQRSTP-------VKV-TDAGD-------------------------------------------------NTKITE------------------------------------------------------NVV----------------QS--------------------------------------TKDV----------YGV--NLSDVPSEDD----------ITIY-ARFVQLGQSQHKQDKSSQ-------------------------------------QFCSK----------------------------------HQL--DGVIN-LIPISSFSQDNIYEVQPPRADRKSIEIFH-AHIQAGKGNMQPLGKDDFLM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYL----------------------* S.townsendi_FIG4_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------VSA----------------P---------------IISSVQKLVLYETRARYFLVGSNHAETKYRVLKIDRTEPKDLVVIDDRHVYTQQEVRELLGRLDLGNRTKIGQKGTS---GLSRAVSAFGIVGFVRFLEG----YYIVLITKRRKMADIG-GHAIYKIEDTNMIYIPNESVRVT--------------------HPDETRY----------------------------------------LRIFQNVDLSSNFYFSYSYDLSHSLQYNLTVLRMPLEILGTETA--------RTRQESFDIFEDEGLAAQGGS--GVFGICSEPYRKYVWNGKLLDVVKT---AVHRDWMLYIIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANCEGDVANEVETEQILCDASVMSFSAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------ARVAALHFDQMLQRFGSPIIILNLVKEREKRKHERILSEELVNAVTYLNQFLPPEHAIVYIPWDMAKYTKSK-LCNVLDRLNVIAENVVKKTGFFVNRPDSYSSVLRPDEKWNELGGCVIPTGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLF-LGVFQPEEGK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHQKDTINFIQARRSYTYWWTPGVLKHLPLPFDEV---------------------------------------------TCPENRRKLT-VK-------KFHKYDEEIDIHNEFFRPYELSSFDDTFCLAMTNSIRDFMPKNVGIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VLGNK------------------------SNREETVLQR--KTAASAPPPPSEE----AVSSSSEDDSGTDKEDEG--------AASQRSTP-------VKM-TDAGD-------------------------------------------------NAKITE------------------------------------------------------NVV----------------QS--------------------------------------IKDA----------YGI--NLSESPSEDD----------LSIY-TRFVQLGQSRHKQDKAHQ-------------------------------------QLCSK-----------------------------------HL--DGVIH-LTHISAFSQDNIYEVQPPKVDRKSVEIFQ-SHIQAGKGIMQPLGKEDVLM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYM----------------------* C.carcharias_fig4a_polyphosphoinositide_phosphatase_isoform_X1 M----------------------------------------P--------------------------------TVQ----------------P---------------VISCIQKLVLYETRARYFLVGSNHAETKFRVLKIDRTEPEDLVVIDDRHVYNQQEVQELLSRLDMGNRTKMGQKGLS---GLSRAVSAFGIVGFVRFLEG----YYIVLITKRRKVADIG-GHSIYKIEDTNIISIPNDSVRIA--------------------HPDEARY----------------------------------------VRIFQSVDLSSNFYFSYSYDLTHSLQFNLTLLKMPSEGLKSEIF-------GHGWQESFDIFEDEGVSTQPGSAPGYYGILNEPYLKYAWNGYILEKVKA---TVHHNWLLYIIHGFCGQSKMLIYG-------------------------RPVYVTLIARRSCKFAGTRFLKRGANCEGDVANEVETEQIIHDASVMSFSAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AHVAALHFDQMFQRFGTPIIILNLVKKREKRKHERILSEALFSAITYLNQFLPPEFFIHYIAWDMAKYTKSK-LCNVLDRLSVIAEDVVKRTGFFVNRPDFYCNSLRPCERWDELGGCIHNTCRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYAIGVIDKPR-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDAINLF-LGVFQPTEGK-SHL--------------------------------------------------------------------------------------------------------------------------------WELPTDYYLHHKNTMALLSPKRSYTHWWTPEVLINLPLPFDEV---------------------------------------------PCTENLKKMTIVK-------KMDKSDEEIDVYTEFFRPYELSCFDDTFCLTMTNSAKDFMPKHVGIDPSPF--------------------------------------------------------------------------------------------T---IRKPEETGKS-MLSNK------------------------SNKEEAVLQR--KTVASAPPPPSEE----VASSSSEDDSGTDKEEEG--------PVSQRSTP-------VKI-TEFGD-------------------------------------------------TTRIPE------------------------------------------------------DST----------------QS--------------------------------------PRET----------HIM--NLSGLPSEDD----------FMIY-KRFVYLGQNQHKVDTYSQ-------------------------------------QNNAK-----------------------------------QL--DNMMTILGPVSLFLQDSIYEVHLADLDRKSAEIFD-SHIQNGKGIIQPMCKDDMIV--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIKNRYM----------------------* X.laevis_fig4.L_polyphosphoinositide_phosphatase_isoform_X2 M----------------------------------------L--------------------------------PAA----------------P---------------IISGIQKLILYETRARYFLVGSNHAETRYRVLKIDRTEPKDLVVIDDKHVYTQQEVRELLGRLDLGNRTKMGQKGSS---GLSRAVSAFGIVGFVQFLEG----YYIVLITKRRKMADIG-GHAIYKIEDTSMIYIPNDSVRVT--------------------HPDEARY----------------------------------------QRIFQNVDLSSNFYFSYSYDLSHSLQYNLTILRMPAEMLKPETV-------PHTRQEGFDIFEDEELSNQGGS--GVFGICSEPYMKYVWNGDLLQNVKD---VVHRDWLLYIIHGFCGQSKLLIYG-------------------------RPVYVTLMARRSSRFAGTRFLKRGANCEGDVANEVETEQIVHDASVMSFTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPITLDQADPF-----------AYAAAFHFDKMLQRFGSPIIILNLVKEREKRKHERILSEELVAAVTYLNQFLPPEHWIVYIPWDMAKYTKSK-LCNVLDRLNVIAESVVKKTGFFVNRSDPYCSVLRPDEKWNELGGVVGQSGRLQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYSLGLIDKPN-LQFDTDAVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDSINLF-LGVFQPAEGK-THL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHHKNTMRLDYAKRSYTFWWTPKVLENLPLPLDEV---------------------------------------------HNSENRKKMT-IK-------KCSKCNEETNIYNEFFRPYELSSFDDTFCLAMTNSARDFMPKSVAIDPSPF--------------------------------------------------------------------------------------------T---VRKPDETGKS-VSGNK------------------------SNKEETLLQR--KTAASAPPPHGEE----AVSSSSEDDSGTDKEDEG--------AVSQRSTP-------VKN-TDTGD-------------------------------------------------HTKASE------------------------------------------------------NYM----------------QS--------------------------------------TKDS----------YGI--NLSDVPSEND----------LQIY-TRFVHLGICQHKQDKRNL-------------------------------------HISSK-----------------------------------PL--ESFAK-LSCISSFSHDNIYEVQPPKVDRKSVEIFT-AHIQAGRGIMQPLGKEDMLT--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIRNRYM----------------------* G.aculeatus_fig4a_polyphosphoinositide_phosphatase_isoform_X1 M----------------------------------------P--------------------------------QSA----------------A---------------TISGIQRMVLYETRARYFLVGTNQAQTKHRVLKIDRTEPKDLVIIDDKHVYNQQEVWELLGRLDLGNRTKIGQKGSS---GLSRSLSAFGIVGFVRFLEG----YYIVLITKRRKMADIG-GHSIYKIEDTSMIYIPNDSVRVT--------------------HPDEARY----------------------------------------VRIFQNVDLSSNFYFSYSYDLSHSLQYNLTLLQRPYELWSSPTSSAEEEVQKQSKHDSFDIFEDEGLPTQV-----VYGLQSEPYYKYVWNGKLLERVKD---IVHRDWLMYIIHGFCGQSKLLIYG-------------------------RPVHITLIARRSSKFAGTRFLKRGANCEGDVANEVETEQIVNDASVMSLTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPIRLDQADPY-----------AHVAALHFDQMLQRFGSPIIILNLVKKREKRKHERILSEELYPAVINLNQFLPPENCIEYLAWDMARYTKSK-LCNVLDRLSMIAENVVKRTGFFSNRSNFYCHTIRPDDRWGDLGGYITASGRVQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYALGMIDKPK-LQFDTDCVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDAINLF-LQVYQPSESR-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHQRNTMALPLDRRSYTMWWSAGVLSYLPVPYDEV---------------------------------------------SCEDTMKKVK-VR-------RANRFEESIDIYTEFFKPYELTSFDDTFCIAMTNSAREFMPKTVGVDPSPF--------------------------------------------------------------------------------------------T---VRKPEETGKS-VLGSK------------------------SSKEETLLQR--KTAASAPPPPSEE----AISSTSEDDSEDDRDDDA--------SVSQRSTP-------IKLLTESGE-------------------------------------------------STRTQE------------------------------------------------------VQVVGELSELRSPPQEIQQQS--------------------------------------VKEP----------YGL--NLAEFPAAKD----------LLIY-ERFAHLGESQHRVERTEH-------------------------------------I---------------------------------------NL--ANIIC-LEPVSCFPEDSIYSLSPPQVDRDSREEFE-SSVLTGRGQVRALCREDMLM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YVKNRYM----------------------* S.salar_fig4a_polyphosphoinositide_phosphatase M----------------------------------------P--------------------------------LSA----------------A---------------VISGVQKLVLYETRARYFLVGSNHAQTKHRVLKIDRTEPKDLAIIDDKHVYNQQEVRELLGRLDLGNRTKMGQKGSS---GLSRAVSAYGIVGFVRFLEG----YYLVLITKRRKMADIG-GHSIYKIEDTNMIYIPNDSVRVT--------------------HPDEARY----------------------------------------VRIFQNVDLSSNFYFSYSYDLSHSLQYNLTLLHSPCDPGTVEEV----HTRSSRQQDSFDIFEDEGLPTQV-----FHGLQSEPYAKYVWNGKLLERVKD---TVHPDWLMYIIHGFCGQSKLLIYG-------------------------RPVYVTLIARRSSKFAGTRFLKRGANCEGDVANEVETEQIIHDASVMSFTAGSYSSYVQVRGSVPLYWSQD-ISTMM-PKPPIRLDQADPY-----------AHVAALHFDQMLQRFGSPIIILNLVKKREKRKHEKILSEELFPAVINLNQFLPPEHCIDYIAWDMARYTKSK-LYNVLDRLSMIAENVVKRTGFFINRSDFFCHTLRPDERWGDLGGSLTSSGRVQTGVLRTNCVDCLDRTNTAQFMVGKCALAYQLYALGMIDKPK-LQFDTDCVRLFEELYEDHGDTLSLQYGGSQLVHRVKTYRKIAPWTQHSKDIMQTLSRYYSNAFSDADRQDAINLF-LQVYQPSESK-PHL--------------------------------------------------------------------------------------------------------------------------------WELPTDFYLHQRNSMALTHDRRSYTYWWSEGILSYLPLPYDEV---------------------------------------------PCEENMKKVG-VR-------RVNRYDESIDTYTEFFKPYELTSFDDTFCIAMTNSARELMPKTVGVDPSPF--------------------------------------------------------------------------------------------T---VRKPEETGKSAVIGAK------------------------SSKEETVVQR--KTAASAPPPPSEE----QISSSSEDDSEDDRDDDG--------SVSQRSTP-------IKLLTESGE-------------------------------------------------SVRTQE------------------------------------------------------DFL---------------QQS--------------------------------------MRET----------YGL--NLAALPAEED----------MLIY-ERFAYLGQNQHKLERTEP-------------------------------------S---------------------------------------VL--ENAIN-VVPVSCFTTDSIYEVSSPQVDRDNREVFE-GHVLTGLGQVRSLCREDILM--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------Y----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------E------------------YIKNRYM----------------------* S.townsendi_SYNJ1_LOW_QUALITY_PROTEIN_synaptojanin1 M----------------------------------------A--------------------------------F------------------------------------SKGYRIYHKLDPPPFSVIVETRNK--EECLMFESGAVAV-LSSAEKEA------------------------IKN---MYSKVMDAYGLLGVLRLNLGETLLHYLVLVTGCMSVGKIQ-ESEVFRVTSTDLISLRID-------------------------PSDEER-----------------------------------------ISEVRKVLNSGNFYFAWSST-GVSLDLSLNAHRSMQE--------------------------------------------QSTDNRFFWNQSLHLHLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACIISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIFL-------DESVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFYTLKNRYGGQI-IVNLLGSKEG---EHMLSKAFQSHLKASE----HADDIKMVNFDYHQMVKGGKAEKLHSVLKPQIQKFLECGFFYFDGKE---------------------VQRYQNGTIRTNCLDCLDRTNSVQAFIGLEMLTKQLEVLGLAEKPQ-L--VTRFQEVFRSMWSVNGDSVSKIYAGTGALEGK-------AKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTSSLR--------ASAKILKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFRNQTLTDWLLDAPKLAGISEFQDRRSKPIDIFAIGFEEMVELNAGNIVSASTTNQKLWAAELQKTISRD--------------------------YKYVLLAAEQLVGVCLFIFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTSSLCFVCS-HFAAGQ------------SQVKERNED----------------FIEICRKLNFPMGRLLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDALLAGDQLVNQKNAGQVFRGFLEGKVAFAPTYKSDVFGVDLLPMVKKIAHQPVGRMGITVLWKRRKWPFDRSAD-HMDLLVCTFHDRGKLLYTWSPGTLLYYGRAELK---TSDHRPVVSLVDIDIFEVDGEERQ-KIYKEVITVQGPPDGTIMVSIKGSSSEENYFDDALIDELLQ-KFATYGEVI----------------------------LIRFV---------------DDK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LN--VLN------------------------------------------------------------------------------------------------------------------------------------------------------------LNGIELLGKTININLKSPDWIKSLEEEMDLEKVNFGLPSSISSTLLCEDAEVSTDYDMEGDIDDYSAEVEEILPQHLQLASGSCLGASPGSSPRSSPCQSPTPSDGPPP---SRPSRTPSKTPGPPISS---QGSPIDFQPVT--QHRESVQPLEPKRPPPPRPVAPPARPAPPQRPPPPSGSKSPAPARKEFGGVGAPPSPVVARREEVHSQSP----------------TGLTSIGTSAPSTSRPTIPPRAGVISAPLSHARSPVGRQTPELQSKPAEPQRGSPMLPEPLKPQTAVPVQPTIQPPTVLKMQDPLIPMTAS-SQTISPQNLEGAPQPPPRSKSSQNLPSEPV--PSQKQVKANGVHDFQLEIKQNNDPFEDLSLCLLPASKIQSTVRVSPVEPLSQKEMTVLPSATDS-FNTLSNVNCMPAT-PVTVCNKSEAQMRTSPNPFATRVNSTNPLTKQTITAGTPFREE---SGVTSHCS-VGH-AAYPFPSLKPPAHHKGNSAFYTNTFQENFNLQSTSSTTNNVKPKGWVTFDE--EDFVLKLKPLENR-TELRQSGSMEATYFPSSLGFEQNHFSSNDSIFNNR-NQ-SADSLHPLPARPAPAPPI--PSRITSTISTTDPLSSLAPKVRSTQDFTER----------------------* F.catus_SYNJ1_synaptojanin1_isoform_X1 M----------------------------------------A--------------------------------F------------------------------------SKGFRIYHKLDPPPLSLIVETRHK--EECLMFESGAVAV-LSSAEKEA------------------------IKG---TYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQ-ESEVFRVTSTEFISLRID-------------------------SSDEDR-----------------------------------------ISEVRKVLNSGNFYFAWSAS-GVSLDLSLNAHRSMQE--------------------------------------------HTTDNRFFWNQSLHLHLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYL-------DDSVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFRTLKNLYGKQI-IVNLLGSKEG---EHMLSKAFQSHLKASE----HAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFVDYGFFYFNGSE---------------------VQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQ-L--VTRFLEVFRSMWSVNGDSISKIYAGTGALEGK-------AKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTGSLRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRD--------------------------NKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLLHTTSLCFVCS-HFAAGQ------------SQVKERNED----------------FVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSDDYDTSEK--CRTPAW--TDRVLWRRRKWPFDRSAE-DLDLLNASFQDGSKILYTWTPGTLLHYGRAELK---TSDHRPVVALIDIDIFEVEAEERQ-NIYKEVIAVQGPPDGTVLVSIKSSVPENNFFDDALIDELLQ-QFTDFGEVI----------------------------LIRFV---------------EDK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LS--VLN------------------------------------------------------------------------------------------------------------------------------------------------------------LNGKELLGRTITITLKSPDWIKNLEEEMSLEKINVPLPSSTSSTLLGEDAEVTADFDMEGDVDDYSAEVEEILPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGHPSS----QSSPMDALPATQLQQK-DSQTLEPKRPPPPRPVAPPTRPAPPQRPPPPSGARSPAPARKEIGGVGAPPSPGAARRETEAPKSPGTTRKENIGRTQPSPQAGLPGPGPAGYSAARPTIPPRAGVISAPQSHARASAGRLAPESQSRPPEGLKGSVLLPEPLKPQAAPPVQPSL-PPPVQKMQEPLVPVAAPLPQSAPQPSLETPPQPPPRSRSSHSLPPEPSPQPPQPQVKTNGVSAVRLESPLKNDPFEDLSFSLLAVSKAQSSIQTSCAPTPNPKGLIQLPSATQSNMNTLSSVTCM---PPIPARSKSQENMRCSPNPFITSLTSTNPFTDRTAAPGNPFRAESQESEATSWLSKDEPVASSPFPSLKPLGHNNSKP-SSFDGFKDSFDLQGQSS-AKISNPKGWVTFEEE-EDFGVKGKSKSSC---------------PDLLSNQPSSLSGS-VTFDDDWNKGTNVPFCVLPSRRPPPPPV--TLPPPATTPPVAPFTTLPSKASPTLDFTER----------------------* E.caballus_SYNJ1_synaptojanin1_isoform_X8 MRKRWACWSGSDASGGCGGSGCGRRRRRSRRKRAASEERRMA--------------------------------F------------------------------------SKGFRVYHKLDPPPFSLIVETRHK--EECLLFESGAVAV-LSSAEKEA------------------------IKG---TYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQ-ESEVFRVTSTEFISLRID-------------------------SSDEDR-----------------------------------------ISEVRKVLNSGNFYFAWSAS-GVSLDLSLNAHRSMQE--------------------------------------------HTTDNRFFWNQSLHLHLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYL-------DDSVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFRTLKNLYGKQI-IVNLLGSKEG---EHMLSKAFQSHLKASE----HAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFDGSE---------------------VQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLTKQLEALGLAEKPQ-L--VTRFQEVFRSMWSVNGDSISKIYAGTGALEGK-------AKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTGSLRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGILEFQDKRSKPTDIFAIGFEEMVELNAGNIVNASTTNQKLWAVELQKTISRD--------------------------NKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCS-HFAAGQ------------SQVRERNED----------------FVEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQIFRGFLEGKVTFAPTYKYDLFSDDYDTSEK--CRTPAW--TDRILWRRRKWPFDRSAE-DLDLLNASFQDGSKILYTWTPGTLLHYGRAELK---TSDHRPVVAMIDIDIFEVEAEERQ-NIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQ-QFTNFGEVI----------------------------LIRFV---------------EDK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LS--VLN------------------------------------------------------------------------------------------------------------------------------------------------------------LNGKELLGRTITITLKSPDWIKNLEEEMSLEKMSVPLPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEEILPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEAPVPSLPIRPSRAPSRTPGHPTSQSTFSGSPVDTLQATQPQQKDSSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGSRSPAPSRKEFGGIGAPPSPGVARREMEAPKSPGTTRKDNIGRNQPSPQAGLAGPGPAGYSAARPTIPPRAGVISAPQSHARASAGRLTPESQSKPPEVLKGSPLLPEPLKPQAALPVQPSL-PPPVQKMQEPLVPVAAPMPQSAPQPYLETPPQPPPRSKSSHGLPSEP---PSQPQVKTNGVSAVRLESPLKSDPFEDLSFNLLAVSKAQLPVHTSLVPTPDLKRLIQSPSATQSNINTLSSASCMPTMPPIPARSKSQENMRCSPNPFITSLSSTNPFTDRTAASGNPFRAESQESEATLWFSKEEPVANTPFLSLKPLGHNKSKPLSSFDGFKDGFDQQGQSM-VKNSNPKGWVTFEEE-EDFGVKGKSKSTC--------------GPDLLGNQPRASSGSSVTFDNDWSKGTNVSFCVLPSRRPPPPPV--PLLPPGTTPPVEPVTTLASKASPTLDFTER----------------------* H.sapiens_SYNJ1_synaptojanin1_isoform_X8 MRKRWACWSGSDAPGGCG-GGCGRRRRRSRRKRAASEERRMA--------------------------------F------------------------------------SKGFRIYHKLDPPPFSLIVETRHK--EECLMFESGAVAV-LSSAEKEA------------------------IKG---TYSKVLDAYGLLGVLRLNLGDTMLHYLVLVTGCMSVGKIQ-ESEVFRVTSTEFISLRID-------------------------SSDEDR-----------------------------------------ISEVRKVLNSGNFYFAWSAS-GISLDLSLNAHRSMQE--------------------------------------------QTTDNRFFWNQSLHLHLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVVYL-------DDSVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFRTLKNLYGKQI-IVNLLGSKEG---EHMLSKAFQSHLKASE----HAADIQMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLDYGFFYFNGSE---------------------VQRCQSGTVRTNCLDCLDRTNSVQAFLGLEMLAKQLEALGLAEKPQ-L--VTRFQEVFRSMWSVNGDSISKIYAGTGALEGK----------AKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTGSLRVSEQTLQSASSKVLKSMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIQEFQDKRSKPTDIFAIGFEEMVELNAGNIVSASTTNQKLWAVELQKTISRD--------------------------NKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTTSLCFVCS-HFAAGQ------------SQVKERNED----------------FIEIARKLSFPMGRMLFSHDYVFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLINQKNAGQVFRGFLEGKVTFAPTYKYDLFSDDYDTSEK--CRTPAW--TDRVLWRRRKWPFDRSAE-DLDLLNASFQDESKILYTWTPGTLLHYGRAELK---TSDHRPVVALIDIDIFEVEAEERQ-NIYKEVIAVQGPPDGTVLVSIKSSLPENNFFDDALIDELLQ-QFASFGEVI----------------------------LIRFV---------------EDK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LN--VLS------------------------------------------------------------------------------------------------------------------------------------------------------------LNGKELLNRTITIALKSPDWIKNLEEEMSLEKISIALPSSTSSTLLGEDAEVAADFDMEGDVDDYSAEVEELLPQHLQPSSSSGLGTSPSSSPRTSPCQSPTISEGPVPSLPIRPSRAPSRTPGPPSA----QSSPIDAQPATPLPQKDPAQPLEPKRPPPPRPVAPPTRPAPPQRPPPPSGARSPAPTRKEFGGIGAPPSPGVARREMEAPKSPGTTRKDNIGRSQPSPQAGLAGPGPAGYSTARPTIPPRAGVISAPQSHARASAGRLTPESQSKTSETSKGSTFLPEPLKPQAAFPPQSSL-PPPAQRLQEPLVPVAAPMPQSGPQPNLETPPQPPPRSRSSHSLPSEA---SSQPQVKTNGISDGKRESPLKIDPFEDLSFNLLAVSKAQLSVQTSPVPTPDPKRLIQLPSATQSN--VLSSVSCMPTMPPIPARSQSQENMRSSPNPFITGLTRTNPFSDRTAAPGNPFRAKSEESEATSWFSKEEPVTISPFPSLQPLGHNKSRASSSLDGFKDSFDLQGQST-LKISNPKGWVTFEEE-EDFGVKGKSKSAC---------------SDLLGNQPSSFSGSNLTLNDDWNKGTNVSFCVLPSRRPPPPPV--PLLPPGTSPPVDPFTTLASKASPTLDFTER----------------------* C.mydas_SYNJ1_synaptojanin1_isoform_X1 MRKSWSGQSGSEAAAVAG-SRSRRRPWRWRRSRAASDKGKMA--------------------------------F------------------------------------SKGYRVYHKLDPPPFSVIVETRNK--EECLMFESGAVAV-LLSAEKEA------------------------IKS---TYSKVMDAYGLLGVLRLNLGDTLLHYLVLVTGCMSVGKIQ-ESEVFRVISTEFISLRSD-------------------------PSDEDR-----------------------------------------ISEVRKVLNSGNFYFAWSAT-GVSLDLSLNAHRSMQE--------------------------------------------HTTDNRFFWNQSLHLPLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIFL-------DDSVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFRTLKNVYGKQI-IVNLLGAKEG---EHMLSKAFQSHLKASE----HAIDIKMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLECGFFYFDGKE---------------------VQRYQSGTIRTNCLDCLDRTNSVQAFFGLEMLTKQLEVLGLAEKPQ-L--VTRFQEVFRSMWSVNGDSVSKIYAGTGALEGK-------AKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTSSLRVSEQSLQSASVKALKGMCENFYKYSKPKKIRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPKLAGIHEFQDRRSKPIDIFAIGFEEMVELNAGNIVNASTTNQKLWAAELQKTISRD--------------------------YKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTSSLCFVCS-HFAAGQ------------SQVKERNED----------------YIEIARKLSFPMGRLLFSHDYIFWCGDFNYRIDLPNEEVKELIRQQNWDSLIAGDQLVNQKNAGQIFRGFLEGKITFAPTYKYDLFSDDYDTSEK--CRTPAW--TDRILWRRRKWPFDRSAE-DLDLLNASFQDESNILYTWNPGTLLHYGKAELK---TSDHRPVVALIDIDIFEVEAEERQ-KIYKEVIAVQGPPDGTVMVSIKSSSPEENYFDDILIDELLQ-KFATYGDVI----------------------------LIRFV---------------EDK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LS--ALN------------------------------------------------------------------------------------------------------------------------------------------------------------LNGTEVLGRIININLKNSDWIRSLEEEMNLEKM-VGLPSSTSSTLLCEDAEVSADYDMEGDIDDYSTEVEEIFPQHLQPGTSSSLRTSPGSSPRSSPCQSPTLSDGPLPTLPVRPSRAPTRTPGPPISS---KCSPVDFQPIT--QQKESTQSLEPKRLPPPRPVAPPTRPAPPQRPPPPSGGRSPAPARKEFGGLGAPPSPGVARREIEVQKSPGTQRKDNLVRSQPPPSAGFTSTGTVGYSAARPTVPPRAGVISAPQSHVRPSVGRPTPETQTKPAEPPRVSPLLPEPLKPQAVVPLQPTIQPPSVQKMQEPLMPMMMHTSQTTPPQNLEAPPQPPPRSRSSHSLPSESV--PSQQQIKANGVYGIKLETQLNNDPFEDLSFNLLTASKVQTSVRISPVPTVNQKELTWLPSATQSNVNSLDTVNCMPAMPPNPAYSKSQEHVRSSPNLLVTRLNSTNPFTERTVNAGNPFRTEMQEPEVTSQLLIEGPAACNPFPSLNHPNPSKGKPMFSISAFEDNFYLQSKSSIVKNTKPKGWVTFEEE-EDSSVKLKTSSECVTEFKRVRSRKPTGFPDLVGTEPNIFPGSDFTFENDWN--SADSFYMLPARRPPAPPV--PSRINQTISPTDPFALLAPKVSPTQDFTER----------------------* G.gallus_SYNJ1_synaptojanin1_isoform_X8 M----------------------------------------A--------------------------------F------------------------------------SKGYRVYHKLDPLPFSVIVEARNR--EECLMFESGAVAV-LSSAEKDT------------------------IKN---TYSKVMDAYGLLGVLRLNLGDTLLHYLVLVTGCMSVGKIQ-DSEVFRVTSTEFVSLRIE-------------------------STDEDR-----------------------------------------ISEVRKVLNSGNFYFAWSAT-GVSLDLSLNAHRSMQE--------------------------------------------HTTDNRFFWNQSLHLHLKHY-GVNCDDWLLRLMCGGVEIRTIYAAH-------------------------KQAKACLISRLSCERAGTRFNVRGTNDDGHVANFVETEQVIYL-------DDSVSSFIQIRGSVPLFWEQP-GLQVGSHRVRMSRGFEA-N-----------APAFDRHFRTLKNLYGKQI-IVNLLGAKEG---EHMLSKAFQSHLKASE----HSADIKMVNFDYHQMVKGGKAEKLHSVLKPQVQKFLECGFFYFDGKE---------------------VKRSQSGTVRTNCLDCLDRTNSVQAFFGLEMLTKQLEVLGLAEKPQ-L--VTRFQEVFRSMWSVNGDSVSKIYAGTGALEGK-------AKAGKLKDGARSVTRTIQNNFFDSSKQEAIDVLLLGNTLNSDLADKAR------ALLTTSSLRVSEQSLQSASVKVLKSMCENFYKYAKPKKVRVCVGTWNVNGGKQFRSIAFKNQTLTDWLLDAPSIAGIHEFQDRRSKPVDIFAIGFEEMVELNAGNIVNASTTNQKLWAAELQKTISRD--------------------------YKYVLLASEQLVGVCLFVFIRPQHAPFIRDVAVDTVKTGMGGATGNKGAVAIRMLFHTSSLCFVCS-HFAAGQ------------SQVKERNED----------------FIEIARKLSFPMGRMLFSHDYIFWCGDFNYRIDIPNEEVKELIRQQNWDSLIAGDQLINQKNSGQIFRGFLEGKINFAPTYKYDLFSDDYDTSEK--CRTPAW--TDRILWRRRKWPFDRSAE-DLDLLNASFHDNSKVPYTWNPGTLLHYGRAELK---TSDHRPVVALIDIDIFEIEAEERQ-KVYKEVIATQGPPDGTVMVSIKSSSAEENYFDDNLIDDLLQ-KFANYGEVI----------------------------LIRFV---------------ADK------------------------------------------------------MWV----------------TF---------------------------------------------------------LEGSSA--LN--VMN------------------------------------------------------------------------------------------------------------------------------------------------------------LNGTEILGRIINISLKNPDWIRSLEDEMNLEKINVGLPSSTSSTLLCEDAEVTADYDMEGDIDDYSAEVEEILPQHLQPTSSSGLGTSPSSSPRSSPCQSPTLSDGP--TLPVRPSRAPAKTPGPPVSV---H---NETQLGT--QQKDTSQTLEPKRPPPPRPVAPPARPAPPQRPPPPSGGRSPAPARKEFGGLGAPPSPGVAKREVEAQKSPGTQRKDNLVRSQPPPSTGFSSAGTVGYAAARPTIPPRAGVISAPQSHVRPSGGRPTPEPQTKPAEPPRGSPLLPEPLKPQAA-PAQPVTQPPPALKMQEPLIPVASHPPQTSAPPSLEPPPQPPPRSRSSHTLPSESA--PSQQQIKTNGTYGTKLETQLNSDPFEDLSFQLL-VSKMQTSARTSAVPTLNQKETMQLPSATQRN-DILNTVSCMPAMPPAPTLNISQEHTRSSPNPFVTGLNSTNPFTERTLTAGNPFRTETREPEITSHLQ-EGHAAFNPFPSLNPSSSNRSKPVFSVDAPENSFCLRSRSLMVKHVQPKGWVTFDEEDENFHVKLKPSKSV-PDFKQIGSRKSAGSPDLLGTEQNTFLGSDFTFGNDWNKSSTDCFYTMPARRPPAPPV--PSRVTSNRSPADPFTSLAPKVSPTQDFTER----------------------* G.aculeatus_sacm1la_phosphatidylinositol3phosphatase_SAC1A_isofor M----------------------------------------G--------------------------------DRREAERMRAVPSLGPMTPRLSSGLKQEAVRFGSAKGLGPSTCLLHTSPEKFFIEACDDGA--DAVLTIDRLSNEM-TLTGRKGL------------------------PPS---TVTR--PICGIMGTVRLVAG----MYLIVITRKRNVGSFL-GHAVWKAVDFDVISYKKTLLHLSEV-----------------QTQENKTF----------------------------------------LSMINNVLMTDGFYFCTDYDLTHTLQRLANTSPDFHE----------------------------------------MSLLERADQRFVWNGNLLRELSA--QPELHRFALPVVHGFIVMKPCRING-------------------------KIFEWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVLY-------EGARASFIQTRGSIPFYWSQRPNLKYK-PKPVITKTTN--H-----------MDGFQRHFDSQLLVYGKQT-ILNLVNQKGS---EKPLEQAFAKMVSDMD-----HAMLNYVAFDFHKECSHMRWDRLQILVDNVAETQEQYSYFMVSPEG--------------------KPLAQQSGLFRSNCMDCLDRTNVIQSLLARRSLQSQLQRMGVLNVGQRIEEQAEFEKIYRNAWADNANACAVQYAGTGALKTDFTRTGKRTRWGLLMDGWNSVIRYYKNNFSDGFRQDSIDLF-LGNFAVDGSD-APT-------------------------------------------------------------------------------------------------------------------------------PLRVQKD--------------------------WKFFTLPIIML---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGAASAITAT--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGQDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VHKEKMD----------------------* S.townsendi_SACM1L_phosphatidylinositol3phosphatase_SAC1 M----------------------------------------A--------------------------------AAA---------------------------------GGAYGNLKLHITPEKFYVEACDDGA--NDVLAIDRVSTEV-TLTVKKDI------------------------PPS---SVTR--QIYGILGTIRLVAG----TYLIVITKKRKVGEFF-NHLVWKAMDFDILSYKKTMLHLTDI-----------------QLQDNKAF----------------------------------------LAMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADPRFVWNGHLLREFAA--QPEIHRFATPVLHGFITMYSCSING-------------------------KYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIAHY-------NGSKASFVQTRGSIPFFWSQRPNLKYK-PKPQISKSVN--H-----------MDGFQRHFDSQIISYGKQV-IVNLVNQKGS---EKPLEQTFSQMVNSLA-----NGMVRYVAFDFHKECSHMRWDRLQILLDRLAEQQDEFGYFLVDADG--------------------KVVIQQEGIFRSNCMDCLDRTNVIQSLLARRSLQAQLQRMGVLHVGQRIEEQAEFEKLYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLVMDSWKSLIRYYKNNFSDGFRQDAIDLF-LGNYSVDEAD-SVS-------------------------------------------------------------------------------------------------------------------------------PFRVQKD--------------------------WKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGSASFGTLA--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* C.mydas_SACM1L_phosphatidylinositol3phosphatase_SAC1_isoform_X1 M----------------------------------------A--------------------------------AT----------------------------------AGAYETLKLHITPEKFYVEACDDGA--DDVLAIDRVSTEV-TLTVKKDV------------------------PPS---AVTR--SIYGILGTIRLVAG----NYLIVITKKKKVGECF-NHAVWKATDFDILSYKKTMLHLTDI-----------------QLQDNKAF----------------------------------------LAMLSHILNVDGFYFSTTYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADPRFVWNGHLLREFAA--QPEIHRFALPVMHGFIAMHSCSING-------------------------KYFDWLLISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------NGSKASFVQTRGSIPFFWSQRPNLKYK-PKPQISKVVN--H-----------MDGFQRHFDSQIISYGKQM-IVNLVNQKGS---EKPLEQTFAKMVNSLG-----NGMVRYIAFDFHKECSRMRWDRLQLLMDQLSEQQDEFSYFLVDSDG--------------------KVVTQQDGIFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQAEFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLF-LGNYSVDEVE-SVS-------------------------------------------------------------------------------------------------------------------------------PLHIQKD--------------------------WKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASFGTLA--IIF------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* C.carcharias_LOC121276208_phosphatidylinositol3phosphatase_SAC1li M----------------------------------------EGMGLVQRSGRRHVSAGVAGVRGQAGGGDGKMAA------------------------------------AAYESLKLHTTPEKFYFEACDDGS--NDVLTIDRVSNEI-TLGVKKDV------------------------PPS---AVTR--SVYGIMGTIRLVAG----MYLIVITKKQKMGELF-NHAIWKATEFNIISYKKTVLHLTEM-----------------QMLDNKTF----------------------------------------LAMLNNVLNTDGFYFSATYDITHTLQRLANTSPEFQE----------------------------------------MTLLERADQRFVWNGHLLREFAA--QPELHRFAVPVIHGFLTVKRCSING-------------------------KYFDWTVLSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVQY-------NGYKVSFVQTRGSIPFFWSQRPNLRYK-PKPQISKSMN--HNIPGQQIALHMMDGFQRHFDSQLISYGKQV-IVNLIDQKGP---EKSLEQVFSKMASSLG-----NGMVKYCAFDFHKECSRMRWDRLQILLDRVADQQDEYGYFLVDTEG--------------------KMLVQQEGTFRTNCIDCLDRTNVVQSLLAHRSLQSQLQRLGVLHVGQRIEEQAEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTKWGLVMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYVVDEAD-SLA-------------------------------------------------------------------------------------------------------------------------------LLHDQKE--------------------------WRFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGSASFGTAA--VIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGKEFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* G.gallus_SACM1L_phosphatidylinositol3phosphatase_SAC1_isoform_1x M----------------------------------------A--------------------------------AAGP---------------A---------------AAASYANLKLHITPEKFYVEACDDGA--DDVLAIDRVSTEV-TLTVKKDV------------------------PPS---AVTR--PIFGILGTIRLVAG----TYLIVITKKKKVGEIF-SHAIWKATDFDILSYKKTMLHLTDI-----------------QLQDNKVF----------------------------------------LSMISHVLSVDGFYFSTTYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADPRFVWNGHLLREFAA--QPEIHRFATPVMHGFISMHSCSING-------------------------KCFDWLLVSRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------KGSKASFVQTRGSIPFFWSQRPNLKYK-PKPQISKSVN--H-----------MDGFQRHFDSQIISYGKQM-IVNLVNQKGS---ELPLEQTFAKMVNSMA-----NGMVRYIAFDFHKECSRMRWDRLQILLDQLAEQQDEFSYFLVDSDG--------------------KIVTQQEGTFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDYTRTGKRTQWGLIMDGWNSLIRYYKNNFSDGFRQDAIDLF-LGNYSVDEVE-SAS-------------------------------------------------------------------------------------------------------------------------------PLHVKKD--------------------------WKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASFGTFA--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD*ICVCGKRLGTEDSSSHTWSLY* D.rerio_sacm1la_phosphatidylinositide_phosphatase_SAC1B M----------------------------------------A--------------------------------N-------------------------------------AYERFNLHSTPEKFYIEACDDGA--DDVLVIDRVSTEM-TLAGIKDI------------------------PPS---GITR--PICGVMGTVRLVAG----MYLIVITRKRKVGDLF-GHTVWKAVEFDVISYKKTILHLTDI-----------------QMQDNKTF----------------------------------------LTMINNVLNTDGFYFCTDYDLTHTQQRLSNTSPDFQE----------------------------------------MSLLERADQRFMWNGNLLREIIA--QPELHKFAFPVIHGFIVMKPCCING-------------------------KVFEWIIISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVQF-------NNARASFVQTRGSIPFFWSQRPNLKYK-PKPLISKDTN--H-----------MDGLRRHFESQVLIYGKQV-ILNLVNQKGS---ELPLEQAFAKMVSSME-----NGFIKYIAFDFHKECSKMRWHRLQILVDAVSDMQEEFGYFMVSSDG--------------------KVLSEQSGTFRSNCMDCLDRTNVIQSLLARRSLQSQLQRMGVLHVGQKIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTHWGLVMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYSVDETD-SLT-------------------------------------------------------------------------------------------------------------------------------PLHVKKD--------------------------WKFLLLPVIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGMASALTAA--VIV------------------------------------------------------------------------------------------------------------------------------------------------------------VNGREFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* S.salar_sacm1la_phosphatidylinositol3phosphatase_SAC1A M----------------------------------------A--------------------------------I-------------------------------------AYERFNLHTSPEKFYIEACDDGA--DAVLAIDRVSNEM-TLTGKKDV------------------------PPS---ANTR--PICGIMGTVRLVAG----MYLIVITKKRNVGSLL-GHAVWKAVDFDVISYKKTVLHLTED-----------------QTQDNKTF----------------------------------------LSMINNVLTTDGFYFCTDYDLTHTLQRLANVSHDFQE----------------------------------------MSLLERADQRFVWNGSLLREFLA--QPELHKFAIPVVHGFVVMKPCRING-------------------------KIFEWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVLY-------NGGRASFVQTRGSMPFFWSQRPNLKYK-PKPLISHNTN--H-----------MDGFQRHFDSQVLIYGKQT-VLNLVNQKGS---EEPLEQAFAKMVTDME-----NGMIKYVAFDFHKECSRMRWDRLCILVDNVAETQDEYSYFMVDTEG--------------------KVLSQQKGTFRSNCMDCLDRTNVIQSMLARRSIQSQLQRMGVLHVAQRIEEQDDFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQMGLVMDGVNSAIRYYKNNFSDGFRQDSIDLF-LGNYAVDETD-GPP-------------------------------------------------------------------------------------------------------------------------------PLRIQKD--------------------------WKFITLPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTAAAVTAA--VIV------------------------------------------------------------------------------------------------------------------------------------------------------------FNGRDFVDS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* S.salar_LOC106570660_phosphatidylinositol3phosphatase_SAC1A M----------------------------------------P--------------------------------T-------------------------------------AYERFNLHTSPEKFYIEACDDGV--EAVLAIDRVSNEM-TLTGKKDV------------------------PPS---ANTR--PICGIMGTVRLVAG----MYLIVITKKRNVGSLL-GHAVWKAVDFDVISYKKTVLHLTEN-----------------QTQDNKTF----------------------------------------LSMINNVLTTDGFYFCTDYDLTHTLQRLANVSHDFQE----------------------------------------MSLLERADQRFVWNGSLLREFLT--QPELHKFAIPVVHGFVVMKPCRVNG-------------------------KIFEWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVLY-------NGGRASFVQTRGSMPFFWSQRPNLKYK-PKPLISKNTN--H-----------MDGFQRHFDSQVLIYGKQT-ILNLINQKGS---EKPLEQAFAKMVTGME-----NSMIKYVAFDFHKECSGMRWDRLSILVDNVAETQDEYSYFMVDTEG--------------------KVLSQQKGTFRSNCMDCLDRTNVIQSMLARRSVQSQLQRMGVLHVAQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQMGLVMDGVNSAIRYYKNNFSDGFRQDSIDLF-LGNYAVDETD-SPT-------------------------------------------------------------------------------------------------------------------------------PLRIQKD--------------------------WKFLTLPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYL----------------LF---------------------------------------------------------WGTAAAVTTA--VIV------------------------------------------------------------------------------------------------------------------------------------------------------------FNGRDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* X.laevis_sacm1l.L_phosphatidylinositol3phosphatase_SAC1_isoform_1 M----------------------------------------A--------------------------------L-------------------------------------AYENLKLHITPEKFYVEACDPGV--EDVLTIDRVSTEV-TLSGKKDI------------------------PPS---AITR--AIYGILGTIRLVAG----MYLIVITRRKKVGDLL-NHSIWKATDFDIISYKKTMLHLTDS-----------------QLQDNKAF----------------------------------------LGMLSHVLSVDGFYFSVSYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADQRFVWNGNLLREFSA--QPEIQKFAIPVVHGFIAIHSCSING-------------------------KYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------NGNKASFVQTRGSIPFYWSQRPNLKYK-PKPQISKAVN--H-----------MDGFQRHFDSQVISYGKQV-VLNLVNQKGS---EKPLEQEFSQMVSGLG-----NGMVRYIAFDFHKECSRMRWDRLQILVEQVAETQDEFGYFLVDTEG--------------------KVVSQQDGIFRSNCMDCLDRTNVVQSLLARRSLQYQLQRLGVLHVGQRIEEQIQFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSLIRYYKNNFSDGFRQDSIDLF-LGNYSVEEAY-STS-------------------------------------------------------------------------------------------------------------------------------PLHIQTD--------------------------WKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASIGTGA--IIM------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD*ICVCGKQLSSDSSVNPVWSLY* F.catus_SACM1L_phosphatidylinositol3phosphatase_SAC1_isoform_X1 M----------------------------------------A--------------------------------A------------------------------------TTYERLKLHITPEKFYVEACDDGA--DDVLVIDRVSTEV-TLSVKKDI------------------------PPS---AVTR--PIFGILGTIHLVAG----NYLIVITKKTKIGEFF-NHVIWKATDFDVLSYKKTMLHLTDI-----------------QLQDNKTF----------------------------------------LAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQE----------------------------------------MSLLERADQRFVWNGHLLRELSA--QPEVHRFALPVLHGFITMHSCSING-------------------------KYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------NGSRASFVQTRGSIPLFWSQRPNLKYK-PLPLINKVAN--H-----------MDGFQRHFDSQVIIYGKQV-IINLVNQKGS---EKPLEQAFATMVSSLA-----SGMIRYVAFDFHKECKNMRWDRLSILMDQVAEMQDELSYFLVDSAG--------------------MVVASQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQHDFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLVMDGWNSLIRYYKNNFSDGFRQDSIDLF-LGNYSVDELE-SHS-------------------------------------------------------------------------------------------------------------------------------PLSVPRD--------------------------LKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGVASIGTFF--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------L------------------VQKEKMD----------------------* D.rerio_sacm1lb_phosphatidylinositide_phosphatase_SAC1A M----------------------------------------A--------------------------------S-------------------------------------TYNSFNLHTTAEKFYIEACDDGV--GDVLAIDRVSTEM-TLTVRKDV------------------------PPS---AVTR--PICGIMGTIRLVAG----VYLIVITKKKKVGDLL-GHAVWKASDFDIISYKKTVLHLTDN-----------------QMQDNKVF----------------------------------------LSMLNSVLNTDGFYFATDYDLTHTLQRLSNTSPEFQE----------------------------------------MTLLERADQRFVWNGHLLREFMA--QPELHRFVFPVIHGFIAMRSCCING-------------------------KIFDWNLISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIIQY-------NGAKASFIQTRGSIPFYWSQRPNLKYK-PKPQISKSIN--H-----------LDGFQRHFDSQIITYGKQV-ILNLVNQKGS---EKPLEQAFAKMVGSLG-----NGMIKYIAFDFHKECSRMRWHRLQILVDTVAELQDEFGYFLVDSDG--------------------SVQMQQDGTFRSNCMDCLDRTNVIQSLLARRSLQSQLERMAVLHVGQRIEEQADFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYAVEEAD-MNT-------------------------------------------------------------------------------------------------------------------------------PLHEPKD--------------------------WKFLTLPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGSASVVTGG--VIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGRDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------L------------------VQKEKMD----------------------* G.aculeatus_LOC120810060_phosphatidylinositol3phosphatase_SAC1B M----------------------------------------A--------------------------------S-------------------------------------TYESFNLRTTPEKFYVEALDEGS--EDVLAIDRVSTET-TLTVRRDI------------------------PAS---AETR--PICGLMGTIRLVAG----MYLVVITKKKKVGDLL-GHVVWKAVDFDIVSYKKTILHLTDT-----------------QMQDNKAF----------------------------------------LSMINNVLHTEAFYFATDYDLTHTLQRLANTSPEFHE----------------------------------------MSLLERADQRFVWNGHLLREFIA--QPELHKFVFPVVHGFITMKSGCISG-------------------------KVFDWIIISRRSCFRAGVRYYVRGIDSEGHPANYVETEQIVQY-------NSAKASFVQTRGSIPFYWSQRPNLKYK-PKPQISKTVN--H-----------LDGFQRHFDSQIILYGRQV-ILNLINQKGS---EQPLELAFDKLVTSLG-----NGMIKYIAFDFHKECSRMRWHRLQILVDMVAEMQDEFGYFLVDADG--------------------KVMSTQEGTFRSNCMDCLDRTNVIQSMLAQRSLQSQLRRMGVLHAGQQIEEQADFGKMFKNAWADNADACAKQYAGTGALKTDFTRTGKRTQWGLLMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYAVDEAD-WAT-------------------------------------------------------------------------------------------------------------------------------PMRDNKD--------------------------WKFLTLPIVMV---------------------------------------------VAFSMCIICL-LMA-GE------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASVVTGG--LIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGLDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKLD----------------------* S.salar_LOC106605518_phosphatidylinositol3phosphatase_SAC1Blike M----------------------------------------A--------------------------------T-------------------------------------AYEKFNLHTTPEKFYIEACDDGS--NDVLSIDRVSTEM-ILTVRKDI------------------------PPH---AVTR--PICGIMGTIRLVAG----TYLIVITKKKKVGDLL-GHAVWKAMDFDVISYKKTVLHLTDN-----------------QMQDNKAF----------------------------------------LSMINSVLLTDGFYFATDYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADQRFVWNGHLLREFMP--QPELHRFAYPVVHGFIIMKSCCING-------------------------KIFDWNIISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVQY-------SGGKASFVQTRGSIPFYWSQRPNLKYK-PKPQISKTID--H-----------LDGFQRHFDSQIIIYGKQV-ILNLIDQKGS---EKQLEQAFDKMVSSLG-----NRMVKYIAFDFHKECSRMRWHRLQILVDMVTEMQDEYGYFLVDVDG--------------------NVLVQQEGMFRSNCMDCLDRTNVIQSLLARRSLQSQLERLGVLHMGQRIEDQADFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLVMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYSIDEAD-MTT-------------------------------------------------------------------------------------------------------------------------------PLHETKD--------------------------WKFLTLPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASFVTAG--VIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGREFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* S.salar_LOC106579366_phosphatidylinositol3phosphatase_SAC1B M----------------------------------------A--------------------------------T-------------------------------------AYEKFNLHTTPEKFYIEACDDGS--NDVLSIDRVSTEM-ILTVRKDI------------------------PPH---AVTR--PICGIMGTIRLVAG----TYLIVITKKKKVGDLL-GHAVWKAMDFDVISYKKTVLHLTDN-----------------QMQDNKAF----------------------------------------LSMINSVLLTDGFYFATDYDLTHTLQRLANTSPEFQE----------------------------------------MSLLERADQRFVWNGHLLREFMP--QPELHRFAYPVVHGFIIMKSCCING-------------------------KIFDWNIISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVQY-------SGGKASFVQTRGSIPFYWSQRPNLKYK-PKPQISKTID--H-----------LDGFQRHFDSQIIIYGKQV-ILNLIDQKGS---EKQLEQAFDKMVSSLG-----NGMVKYIAFDFHKECSRMRWHRLQILVDMVTEMQDEYGYFLVDVDG--------------------NVLVQQEGMFRSNCMDCLDRTNVIQSLLARRSLQSQLERLGVLHMGQRIEDQADFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQWGLVMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYAIDEAD-MTT-------------------------------------------------------------------------------------------------------------------------------PLHETKD--------------------------WKFLTLPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGTASFVTAG--VIL------------------------------------------------------------------------------------------------------------------------------------------------------------FNGREFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKMD----------------------* E.caballus_SACM1L_phosphatidylinositide_phosphatase_SAC1_isoform_ M----------------------------------------A--------------------------------A------------------------------------TTYEQLKLHITPEKFYVEACDDGA--DDVLIIDRVSTEV-TLSVKKDI------------------------PPS---AVTR--PIFGILGTIHLVAG----NYLIVITKKTKIGEFF-NHVIWKATDFDVLSYKKTMLHLTDI-----------------QLQDNKTF----------------------------------------LAMINHVLSVDGFYFSTTYDLTHTLQRLSNTSPEFQE----------------------------------------MSLLERADQRFVWNGHLLRELSA--QPEVHRFALPVLHGFITMHSCSING-------------------------KYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------NGSKASFVQTRGSIPVFWSQRPNLKYK-PLPQINKVAN--H-----------MDGFQRHFDSQVIIYGKQV-IINLVNQKGS---EKPLEQAFATMVSSLA-----SGMIRYVAFDFHKECKNMRWDRLSILLDQVAETQDELSYFLVDPVG--------------------KVVTNQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTQLGLIMDGWNSLIRYYKNNFSDGFRQDSIDLF-LGNYSVDELE-SHS-------------------------------------------------------------------------------------------------------------------------------PLSVPRD--------------------------LKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGVASIGTFF--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K------L------------------VQKEKID----------------------* H.sapiens_SACM1L_phosphatidylinositol3phosphatase_SAC1_isoform_1x M----------------------------------------A--------------------------------T------------------------------------AAYEQLKLHITPEKFYVEACDDGA--DDVLTIDRVSTEV-TLAVKKDV------------------------PPS---AVTR--PIFGILGTIHLVAG----NYLIVITKKIKVGEFF-SHVVWKATDFDVLSYKKTMLHLTDI-----------------QLQDNKTF----------------------------------------LAMLNHVLNVDGFYFSTTYDLTHTLQRLSNTSPEFQE----------------------------------------MSLLERADQRFVWNGHLLRELSA--QPEVHRFALPVLHGFITMHSCSING-------------------------KYFDWILISRRSCFRAGVRYYVRGIDSEGHAANFVETEQIVHY-------NGSKASFVQTRGSIPVFWSQRPNLKYK-PLPQISKVAN--H-----------MDGFQRHFDSQVIIYGKQV-IINLINQKGS---EKPLEQTFATMVSSLG-----SGMMRYIAFDFHKECKNMRWDRLSILLDQVAEMQDELSYFLVDSAG--------------------QVVANQEGVFRSNCMDCLDRTNVIQSLLARRSLQAQLQRLGVLHVGQKLEEQDEFEKIYKNAWADNANACAKQYAGTGALKTDFTRTGKRTHLGLIMDGWNSMIRYYKNNFSDGFRQDSIDLF-LGNYSVDELE-SHS-------------------------------------------------------------------------------------------------------------------------------PLSVPRD--------------------------WKFLALPIIMV---------------------------------------------VAFSMCIICL-LMA-GD------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------TWTETL------------------------------------------------------AYV----------------LF---------------------------------------------------------WGVASIGTFF--IIL------------------------------------------------------------------------------------------------------------------------------------------------------------YNGKDFVDA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------P----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------R------L------------------VQKEKID*ICICGKRLGLEDSIVQNWSLY* C.mydas_INPP5F_phosphatidylinositide_phosphatase_SAC2_isoform_X2 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQRGEL------ALWCSRRDGTL-QLRPATDL------------------------LLAWNPVCLG--LVEGVIGKVQLHTDL--PWWLLLIRQKALIGKLPESHEVCKITKIAVIPLSETDPQDLELELCKKHHFGINKTEKITQSPDDSKFLLKTFTQIKSNVSASNKKK------IKESKEKEKLERRLLEELFKMFMDSDSFYYSLTYDLTNSVQKQSSC--EKTN----------------------------------------LSLWRKVDDRFFWNKHMIQDLINTDNAEVDSWIIPIIQGFVQIEELVVNYSDASDDEKSSPETPPQESICVDDIHPRFLVALISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHV-------HNHTLSFIQTRGSVPVFWSQV-GYRYN-PRPRLDKSEKE-T-----------VPYFCAHFEEQLKIYKKQV-IINLVDQTGR---EKIIGDAYLKQVLLYN-----NSNLTYVSFDFHEHCRGMKFENVQTLTDAIHDIILDMKWCWVDQAG---------------------VICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPL--PMKCNRIYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQAVIDLM-QGIPVTEDLY-SIFTKEKEHEALHKETLR--------SHQELISQLLQSYM------------------------------------------------------------------------------------------KLLLPDDEK---------------FHGG-----WALIDCDPSLI---------------------------------------------DATHKDVDVL-LLL-SNSSYYVAYYDDEVDKVNQYQRL----------------SLEALEKIEIGPEPTLFGKP-KFSCMRLHYKYNETSGY---------------------------------------------------FHT--------------LRAV---VRNPEEDGKD-TLQCI------------------------AEML---RIT--KQAMGLDVPIIEKKLERKSSKPHEDIIGIRSQNRG--------SLAQGKNF-------LMS-KFSSLNQKVKQTKSNVNIGNLR---KLGNF--TKPEMKVNFLKPNLKVNLWKSDSSLETLENPMA--DTKVCAESDIEI-SDNDSFHSD-DFLTNSKSDEDHQLTGSLENVGQIDYV----------------LPSCGIIASAPRLGSRSQSINSTDINISIHIPSEIHV-TQANRP----------ECEDRPMQGSPFADNV--AMEFIKPIDAYCHRFVQDAQNKMTDASMAETAPQQS---CQRVDQTSNSAS-----QKTETWSETNRDIPSRPSKLDVPFSESGSQFLAVEEIYSNRSHKSPGSAPSILEVETGLH-MTPSP--ADSNGSRAVSPFAKIRSSMVQVANITQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSPAEPE--AVNDVQQNELIEMFTQC------QTRIIQI----------------------* G.gallus_INPP5F_phosphatidylinositide_phosphatase_SAC2_isoform_X2 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQSGER------ALWCSRRDGSL-QLRAATDL------------------------LLAWNPICLG--LVEGVIGKVQLHTDL--PWWLLLIRQKALIGKLPGDHEVYKITKVAVIPLSETEPQDLELELCKKHHFGINKPEKITQSPDDSKFLLKTLTQIKSNVSAPNKKK------IKESKEKERLEKRLLEELFKMFMDSDSFYYSLTYDLTNSVQRQSAC--EKTD----------------------------------------LPLWRKVDDRFFWNKHMIEDLIITDNTEVDFWIMPIIQGFVQIEELVVNYSESSDDEKSSPETPPQESTCVDDIHPTFLVVLISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHV-------HNHTLSFVQTRGSVPVFWSQV-GYRYN-PRPRLDKSENE-T-----------VACFRAHFEEQLKNYRKQV-IINLVDQTGR---EKIIGDAYLKQVLLYN-----NANLTYVSFDFHEHCRGMKFENVQTLTDAIHDIILDMKWCWVDQAG---------------------VICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPL--PVKCNRIYQIIWANNGDAISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQAVIDLM-QGIPVTEDLY-SIFTKEKEHEALHKENLR--------SHQELISQLLQSYM------------------------------------------------------------------------------------------KLLLPDDEK---------------FHGG-----WALIDCDPSLI---------------------------------------------DATHKDVDVL-LLL-SNSAYYVAYYDDEIDKVNQYQRL----------------SLEALEKIEIGPEPTLFGKP-KFSCMRLHYKYKDTSGY---------------------------------------------------FHT--------------LRAV---VRNPDEDGKD-TLQCI------------------------AEML---RIT--KQAMGLDVPIIEKKLERKSSKPHEDIIGIRSQNRG--------SLAQGKNY-------LLS-KFSSLNQKVKQTKSNVNISNLR---KLGSF--TKPEVKVNFLKPNLKVNLWKSDSSLETLENPAV--DTKVHAESDTEM-SDNDSFHSD-DFLTNSKSDEDNQLTDSLENIGQIDYV----------------LPSCGIIASAPRLGSRSQSISSTDMNISIHVPSEIHV-TQANQS----------DGEDVPV---PSADNA--EMEFAKPIDVYCQRFVQDAQNKMSDALEAETCSEEP---RHVINETSNNVC-----KKAENKTETIGGIPSRPSKLDVQSE-PNPQLLAVHGTDFTKSHRSPGSASGIL--ETGLH-TTPSP--ADSSSSRAVSPFAKIRSSMVQVANITQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSPAESE--ALNEIKQKELKEMFTQC------QTRIIQI----------------------* F.catus_INPP5F_phosphatidylinositide_phosphatase_SAC2_isoform_X1 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQQGER------ALWCSRRDGGL-QLRPATDL------------------------LLAWNPICLG--LVEGVIGKIQLHSDL--PWWLILIRQKALVGKLPGDHEVCKVTKIAVLSLSEMEPQDLELELCKKHHFGINKPEKIVPSPDDSKFLLKTFTHIKSNVSAPNKKK------VKESKEKEKLERRLLEELLKMFMDSESFYYSLTYDLTNSVQRQSTG--ERDP----------------------------------------RPLWQKVDDRFFWNKYMIQDLTEIGTPDVDFWILPIIQGFVQIEELVVNYNESSDDEKSSPETPPQESTCVDDIHPRFLVALISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHV-------HNHTLSFIQTRGSVPVFWSQV-GYRYN-PRPRLDKSEKD-T-----------IAYFCAHFEEQLKIYKKQV-IINLVDQAGR---EKIIGDAYLKQVLLFN-----SSHLTYVSFDFHEHCRGMKFENVQTLTDAIYDIILDMKWCWVDQAG---------------------VICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPL--PVKCNRIYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFKDAYRQAVIDLM-QGIPVTEDLY-SIFTKEKEHEALHKENQR--------SHQELISQLLQSYM------------------------------------------------------------------------------------------KLLLPDDEK---------------FHGG-----WALIDCDPSLI---------------------------------------------DATHRDVDVL-LLL-SNSAYYVAYYDDEVDKVNQYQRL----------------SLEDLEKIEIGPEPTLFGKP-KFSCMRLHYRYKEASGY---------------------------------------------------FHT--------------LRAV---MRNPEEDGKD-TLQCI------------------------AEML---QIT--KQAMGLDVPIVEKKLERKSSKPHEDIIGIRSQNQG--------SLAQGKNF-------LMS-KFSSLNQKVKQTKSNVNIGNLR---KLGNF--TKPEMKVNFLKPNLKVNLWKSDSSLETMENTGV-MDNKVQAESDGEMSSDNDSYHSD-EFLTNSKSDEDRQLANSLENVGPIDYV----------------LPSCGIIASAPRLGSRSQSLSSTDIS--IHVPSEGAA-HGSGFG----------KGQESPLKKSPSADNIHVLTGFAKPVDIYCHRFVRDAQNKMTRPSEAGSVSQQA---SEEGNQVTNQVS-----N-EETPSESTEPLPSRPSQLDVASSASGPQYLSVEPVHPVGSQKTPGSGSSTRELETGLP-VTPSP--SEGAGSRAVSPFAKIRSSMVQVASITQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSPAESE--VVQEVRQNELKNMFTQC------QTRIIQI----------------------* H.sapiens_INPP5F_phosphatidylinositide_phosphatase_SAC2_isoform_1 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQQGER------ALWCSRRDGGL-QLRPATDL------------------------LLAWNPICLG--LVEGVIGKIQLHSDL--PWWLILIRQKALVGKLPGDHEVCKVTKIAVLSLSEMEPQDLELELCKKHHFGINKPEKIIPSPDDSKFLLKTFTHIKSNVSAPNKKK------VKESKEKEKLERRLLEELLKMFMDSESFYYSLTYDLTNSVQRQSTG--ERDG----------------------------------------RPLWQKVDDRFFWNKYMIQDLTEIGTPDVDFWIIPMIQGFVQIEELVVNYTESSDDEKSSPETPPQESTCVDDIHPRFLVALISRRSRHRAGMRYKRRGVDKNGNVANYVETEQLIHV-------HNHTLSFVQTRGSVPVFWSQV-GYRYN-PRPRLDRSEKE-T-----------VAYFCAHFEEQLNIYKKQV-IINLVDQAGR---EKIIGDAYLKQVLLFN-----NSHLTYVSFDFHEHCRGMKFENVQTLTDAIYDIILDMKWCWVDEAG---------------------VICKQEGIFRVNCMDCLDRTNVVQAAIARVVMEQQLKKLGVMPPEQPL--PVKCNRIYQIMWANNGDSISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFKDAYRQAVIDLM-QGIPVTEDLY-SIFTKEKEHEALHKENQR--------SHQELISQLLQSYM------------------------------------------------------------------------------------------KLLLPDDEK---------------FHGG-----WALIDCDPSLI---------------------------------------------DATHRDVDVL-LLL-SNSAYYVAYYDDEVDKVNQYQRL----------------SLENLEKIEIGPEPTLFGKP-KFSCMRLHYRYKEASGY---------------------------------------------------FHT--------------LRAV---MRNPEEDGKD-TLQCI------------------------AEML---QIT--KQAMGSDLPIIEKKLERKSSKPHEDIIGIRSQNQG--------SLAQGKNF-------LMS-KFSSLNQKVKQTKSNVNIGNLR---KLGNF--TKPEMKVNFLKPNLKVNLWKSDSSLETMENTGV--MDKVQAESDGDMSSDNDSYHSD-EFLTNSKSDEDRQLANSLESVGPIDYV----------------LPSCGIIASAPRLGSRSQSLSSTDSS--VHAPSEITVAHGSGLG----------KGQESPLKKSPSAGDVHILTGFAKPMDIYCHRFVQDAQNKVTHLSETRSVSQQA---SQERNQMTNQVS-----N--ETQSESTEQTPSRPSQLDVSLSATGPQFLSVEPAHSVASQKTPTSASSMLELETGLH-VTPSP--SESSSSRAVSPFAKIRSSMVQVASITQAG--L--THGINFAVS--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSPPEPE--IINQVQQNELKKMFIQC------QTRIIQI----------------------* S.salar_inpp5f_phosphatidylinositide_phosphatase_SAC2 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQSGDH------ALWCSRIDGTM-NVRPATDL------------------------LLARNPTCLG--LVEGLVGKIQLHTDL--PLGLILIRQKVLVGQMPGDHKVYKITKIAVIPLSEEEPLELELELCKKHHFGIDKPEKLAQSPDESKFLMKTLSQIKSNVGAPTKKK------VKENKEKERLERRLLDELYKIFMDSDSFYYSMTYDLTNTVQRQGDT--EKSN----------------------------------------LPLWNQVDDRFFWNKHMVQELIDLQVPEVDFWVTPIIQGFVQVEELVVNYNESPDEEKNSPDTPLQELTCVDDIHPRFTVALISRRSRHRAGMRYKRRGVDTDGHVANFVETEQLIHV-------HNHSLSFVQSRGSVPVFWSQA-GYRYN-PRPRLEKGEKE-T-----------IPYFAAHFDEQLNLYKKQV-IINLVDQSGR---EKIIGDAYLKQVLLYN-----NPNLTYVSFDFHEHCRGMKFENVQTLTDAISDIIADMRWGWVDQAG---------------------VICHQEGIFRVNCMDCLDRTNVVQASFARAVMEAQLKKLGVMPPEQPL--PLKCYRIYQVMWANNGDTISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQAVIDLM-MGHPVTEDLY-SIFSKEKEHEENEQESQR--------VTQEQVSLLLQTYM------------------------------------------------------------------------------------------QLLLPDDEK---------------FHGG-----WALIDCDMSLI---------------------------------------------DATSKDVDVL-LLL-SNSAYYVAYYDEEADKVNQYQRL----------------NLEGLEKIEIGPEPTLFGKP-KFCCMRLHYNNGETSGY---------------------------------------------------FHT--------------LRAL---TRNPEDDGKD-TLQCI------------------------AEML---RLT--KQAMGLDLQVVEKRLERRHSKPHEDIMGIQGRHPDQVLGS--SGLAQGKSF-------LLN-KFSSLNKRVK--KSNVNMGSFK--GRLGNTFSTKPDVKVNFLKPTMGVTLWKSDSSLETSDGANIGPALKDLCNNHSEMSSDSDSYNSD-EQL----------RSSSLEN---VDYV----------------LPSCGIVASAPRLGSRG----SVELN--------IRV-TGCDSNSKQEAPALASPEDQSPGVTSEAEEA--ILIDFGTPIDAYCHQFIQDAQTKPVEVFGEQVACSLPTQNPQVPPHAKGPLAPDEHPG--SDQQEEELQLPPRPSQLDVAS---GPNLLTVQKPISAASGSSQRSLGSHM--EG--S-LGPSP--ADSNGSRVVSPFAKIKSSMVQVASLTQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSP-EPEMATLNETQENELKAMFTQC------QTRIIQI----------------------* S.salar_LOC106604534_phosphatidylinositide_phosphatase_SAC2like_i M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQSGDH------ALWCSRIDGTM-NARPATDL------------------------LLAWNPVCLG--LVEGLIGKIQLHTDL--PLGLILIRQKALVGQMPGDHKVYKITKIAVIPLSEEEPLDLELELCKKHHFGIDKPEKLAPSPDESKYLMKTLSQIKSNVGAPTKKKYSPAFQVKENKEKERLERRLLDELYKIFMDSDSFYYSMTYDLTNTVQRQGDT--EKSN----------------------------------------LPQWKQVDDRFFWNKHMVQELIDQQVPEVDFWVTPIIQGFVQVQELVVNYNESPEEEKSSPDTPLQEHTCVDDIHPRFTVALISRRSRHRAGMRYKRRGVDTDGHVANFVETEQLIHV-------HNHSLSFVQSRGSVPVFWSQA-GYRYN-PRPRLEKGEKE-T-----------IPYFAAHFDEQLNLYKKQV-IINLVDQSGR---EKIIGDAYLKQVLLYN-----NPNLTYVSFDFHEHCRGMKFENVQTLTDAISDIIADMRWGWVDQAG---------------------VICHQEGIFRVNCMDCLDRTNVVQAAIARAVMEAQLKKLGVMPPEQPL--PLKCYRIYQVMWANNGDTISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQAVIDLM-MGHPVTEDLY-SIFSKEKEHEEKEQESQR--------VTQEQVSLLLQTYM------------------------------------------------------------------------------------------QLLLPDDEK---------------FHGG-----WALIDCDMSLI---------------------------------------------DATSKDVDVL-LLL-SNSAYYIAYYDEEADKVNQYQRL----------------NLEGLEKIEIGPEPTLFGKP-KFCCMRLHYKNEETSGY---------------------------------------------------FHT--------------LRAV---TRNPEDDGKD-TLQCI------------------------AEML---CIT--KQAMGLDMQVIEKKLERRHSKPHEDIMGR---HPDQVLGS--SGLAQGKSF-------LLN-KFSSLNQRVK--KSNVNMGSFK--GRLGNT--FSTKPDVNFLKPTIGITLWKSDSSLETSEGANTGPALKDLCDNHSEMSSDSDSYNSD-EHL----------RSGSLEN---VDYV----------------LPSCGIVASAPRLGSVELNI---------------RV-TGCDSK--QGAPALAGPEDQSPDATSEGEEA--ILIDFGTPIDAYCHQFIQDAQIKPVEVFGEQMTGGVPTQNPNVPSHAKGPLAPDEHPGSDQKVQAKELQLPPRPSQLDVTS---RPNLLTAQKLSSAASGSSQRSLGSHM--EG--S-LGSLP--TNGNGSRVVSPFAKIKSSMVQVASLTQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSP-EPEMAGLNETQENELKAMFTQC------QTRIIQI----------------------* D.rerio_inpp5f_phosphatidylinositide_phosphatase_SAC2_isoform_X1 M--------------------------------------------------------------------------------------------------------------------ELFQAKDHYILQSGDN------ALWCSRKDGSM-AVRPATDL------------------------LLAWNPVCLG--LVEGIIGKMQLHVDL--PLGLILIRQKALVGQLPGDHKVYKITKIVVIPLSEDEPQDLELELCKKHHFGINKPERITQSPDETKFLMKTLSQIKSNVGAPIKKKYSPAFQVKENKEKERLERRLLDELYKIFMDSDSFYYSLTYDLTNTVQRQGEL--GKSD----------------------------------------QPLWKRVDDRFFWNKHMIKDLVDLQAPQVDFWVIPIIQGFVQVEELVVNYNESSDEERSSPETPLQEPTCVDDIHPRFTVALISRRSRHRAGMRYKRRGVDTDGHVANYVETEQLIHV-------HSHTLSFVQTRGSVPVFWSQA-GYRYN-PRPRIEKGERE-T-----------MPYFASHFEKEVETYKKLV-IINLVDQNGR---EKIIGDAYLKQVLLYN-----NPNLTYVSFDFHEHCRGMKFENVQTLTDAIYDIITDMRWAWVDQAG---------------------VICQQEGIFRVNCMDCLDRTNVVQAAIARVIMEQQLKKLGVMPPEQPL--PLKCYRIYQVMWANNGDTISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQAVIDLM-MGHPVTEDLY-SIFSKEKEHEEKEKESQR--------GAQEQVSLLLQTYM------------------------------------------------------------------------------------------QLLLPDDEK---------------FHGG-----WALIDCDPSLI---------------------------------------------DATHKDVDVL-LLL-SNSAYYVAYYDEEADKVNQYQRL----------------SLEGLEKIEIGPEPTLFGKP-KYCCMRLHYKNGETSGY---------------------------------------------------FHT--------------LRSV---TRNPEDDGKD-TLQCI------------------------AEML---RIT--KQAMGLDVQVVEKKLERRHSKPHEDIMGIQGKAVDQVLGS---GLAQGKSF-------FLN-KFSTLNQKVKQTKTNVNIGNFKPLGKLGTF--SKPEVKVNFLKPNLHMNLWKSDSSLETHDSNTGSGALKDHGPHSEEISSDSDSYNSD-EQP----------CSGSREN---VDYV----------------LPSCGIVASAPRLGSRSQSIGSVEIA----VPSVIRV-TGCDEK--T-MDSLSVAADQSPGAASEAEEA--ILIDFGTPIDVYCHQFVQDAKTKPIEVFEEVAPAPKP-----QGPQVP--LAPDAKLG----SSHSQ-NQLPRPSQLEVESNVHGANLLTVQPVGSATSCGSQK----SL--EG--I-TGPSS--ADSNGSRVVSPFAKIRSSMVQVASLTQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSP-EPD--AVNETQENELRAMFTQC------QTRIIQI----------------------* G.aculeatus_inpp5f_phosphatidylinositide_phosphatase_SAC2_isoform M--------------------------------------------------------------------------------------------------------------------ELFQAKDDYILQRGDR------ALWCSRKDGTV-TVRPATDL------------------------LLAWNPVCLG--LVEGVIGKIQLHTDL--PLGLVLIRQKALVGNLPGGHKVYKITKIAVIPLSDEEPQELELELCKKHHFGIDKPEKLVQSPDESKFLMKTFSQIKSNVAVPIKKKYSPAFQVKENKEKERLERRLLDELYKVFMDSDSFYYSMTYDLTNSVQRQGDS--EKSS----------------------------------------LPLWKRVDDRFFWNKHMIQDVIDLQVPEVDLWVIPIIQGFVQVEELVVNYNETPDEERSSPETPPQEVTCVDDIHPRFTVALISRRSRHRAGMRYKRRGVDTDGHVANYVETEQLIHV-------HSHTLSFVQTRGSVPVFWSQA-GYRYN-PRPRLEKGEKE-T-----------MSYFSAHFEEQLKLYKTQV-ILNLVDQSGR---EKIIGDTYLKQVLLYN-----NPNLTYVSFDFHEHCRGMKFENVQVLTDAISDIITEMKWAWVDQAG---------------------VICKQEGIFRVNCMDCLDRTNVVQAAIARAVMEQQLKKLGVMPPEQPL--PPKCYRIYQIMWANNGDTISRQYAGTAALKGDFTRTGERKLAGVMKDGVNSANRYYLNRFRDAYRQSVIDLM-MGLPVTEDLY-SIFSKEKEHEEKEKESLR--------GAQEQVGLLLQTYM------------------------------------------------------------------------------------------QLLLPDDET---------------FHGG-----WALINCDMSLV---------------------------------------------DANNKDVDVL-LLL-SDKAYYIAYYDEEADKVDQYQRL----------------NLEGLEKIEIGPEPTLFGKP-KFCCMRLHYRSEEMSGY---------------------------------------------------FHT--------------LRAA---TRNPDDDGKD-TLQCI------------------------AEML---RIT--KQATGLDLLVIEKRLDRRQSKPHEDIMGIQNKPADPVQGS--SGLAQGKSF-------LLN-KFSSLNQKVKQTKTNVNMGPFKPLGRLGNF--SKPDVKVNFLKPTMHVNLWKSDSSLETSDNNPGTAALKGIG---DRLSDDSDSYNSDPEHP----------CSGSLEN---VDYV----------------LPSCGIVASNPRLGSRSQSIGSVELN----IPSVIRV-TGCDGK--Q-EGLSQGSDENSPGSASVAEEA--ILIDFGTPIDAYCHQFVQDAQTKPVEVFGEQPPAAAPPPNPGARVPNKSPADSDRQARRPEPRHEPEEPQLPRPSQLDVDSAPPSSNLLSVQKPGSGGS---QRSLCSQV--EG--S-LGPSP--ADSNGSRVVSPFAKIKSSMVQVASLTQAG--L--TQGINFAVA--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------K--------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------VQKSP-EPD--AVNEAQENELKAMFTQC------QTRIIQI----------------------* 0^ 20^ 40^ 60^ 80^ 100^ 120^ 140^ 160^ 180^ 200^ 220^ 240^ 260^ 280^ 300^ 320^ 340^ 360^ 380^ 400^ 420^ 440^ 460^ 480^ 500^ 520^ 540^ 560^ 580^ 600^ 620^ 640^ 660^ 680^ 700^ 720^ 740^ 760^ 780^ 800^ 820^ 840^ 860^ 880^ 900^ 920^ 940^ 960^ 980^ 1000^ 1020^ 1040^ 1060^ 1080^ 1100^ 1120^ 1140^ 1160^ 1180^ 1200^ 1220^ 1240^ 1260^ 1280^ 1300^ 1320^ 1340^ 1360^ 1380^ 1400^ 1420^ 1440^ 1460^ 1480^ 1500^ 1520^ 1540^ 1560^ 1580^ 1600^ 1620^ 1640^ 1660^ 1680^ 1700^ 1720^ 1740^ 1760^ 1780^ 1800^ 1820^ 1840^ 1860^ 1880^ 1900^ 1920^ 1940^ 1960^ 1980^ 2000^ 2020^ 2040^ 2060^ 2080^ 2100^ 2120^ 2140^ 2160^ 2180^ 2200^ 2220^ 2240^ 2260^ 2280^ 2300^ 2320^ 2340^ 2360^ Number of sequences: 42 Alignment width: 2364 Sequence type: aa